Genetic Diversity of Selected Gladiolus (Gladiolus × Gandavensis Van Houtte) Cultivars Assesed by Microrna-Based Markers

Gladiolus as economically important flowering plants reflect the wide range of morphological variability inflorescences. The aim current gladiolus breeders is to create interesting new genotypes by different breeding techniques, carrying a variety colour, size, texture and shapes flowers Despite well-established characterization germplasm, genomic screening functional markers can provide useful information for breeders. miRNA-based assay was conducted on 9 (Gladiolus × gandavensis Van Houtte) hybrids individual following families: miR156, miR160, miR398, miR408, miRr414 miR482. A total 291 loci were amplified, which marker miR408 most abundant (25%), with representation other types: 21% – miR414; 19% miR156; 16% miR160; 12% miR482 7% miR398. Genetic diversity selected cultivars assessed two markers, lus-miR408 hyp-miR414, provided genotype-specific profiling in form molecular fingerprinting. Stress-sensitive miR398 specifically amplified genotype Správna Susane, susceptible water deficit all analysed genotypes. Marker miR141 able distinguish Athos Dandy among themselves, but also from cultivars.